ExoQuick CG

How It Works ?

Exosome Size Distribution and Yields Using ExoQuick-CG

Figure 1. ExoQuick-CG efficiently isolates exosomes from conditioned cell culture media.

3.3 mL of ExoQuick-CG was added to 10 mL of conditioned media collected from human HT1080 lung sarcoma cells or mouse JAWS II dendritic cells cultured in Exo-FBS (exosome-depleted FBS medium supplement; Cat. No. EXO-FBS-50A-1). Samples were incubated overnight at 4 °C to allow exosome precipitation. Following centrifugation, exosome pellets were resuspended in 0.5 mL PBS and analyzed using a NanoSight LM10 instrument for visualization and particle assessment.

Exosome CD63 Western Blot Validation Studies

Figure 2. Western blot confirmation of exosome marker CD63 in ExoQuick-CG–isolated samples.

Approximately 10–50 µg of exosomal protein, obtained from the NanoSight analyses shown in Figure 1, was resolved by denaturing SDS-PAGE and analyzed by Western blotting. The exosome-specific marker CD63 was detected using SBI’s rabbit anti-CD63 primary antibody and an HRP-conjugated goat anti-rabbit secondary antibody (Cat. No. EXOAB-CD63A-1), confirming successful isolation of CD63-positive exosomes.