Exo-Fect Transfection Kit (20 rxns)
xo-Fect™ enables the efficient loading of RNAs, DNAs, and small molecules into isolated exosomes, transforming them into customizable delivery vehicles for functional studies and translational research. Designed for simplicity and versatility, Exo-Fect™ provides a fast, reliable alternative to complex electroporation-based loading methods.
Putting exosomes to work
See how Exo-Fect can be used to create cargo-delivering exosomes
Figure 1. Exo-Fect™ enables efficient loading of siRNA into exosomes.
Fluorescence microscopy images show exosomes loaded using Exo-Fect™ with either a Texas Red end-labeled siRNA (left panels) or unlabeled siRNA (right panels). Following loading, exosomes were captured on Exo-Flow™ CD63 magnetic beads and immobilized for imaging, demonstrating successful incorporation of siRNA cargo into exosomes.
Figure 2. Exo-Fect™-loaded exosomes successfully deliver siRNA cargo to recipient cells.
Exosomes loaded with siRNA using Exo-Fect™ (as shown in Figure 1) were eluted from Exo-Flow™ CD63 magnetic beads and subsequently incubated with HEK293 cells. Fluorescence detected in recipient cells confirms the successful delivery and cellular uptake of the Texas Red–labeled siRNA cargo mediated by Exo-Fect™–transfected exosomes.
Exo-Fect efficiently loads mRNAs and DNA plasmids into exosomes for delivery to recipient cells
Figure 3. Exo-Fect™-loaded exosomes deliver mRNA and plasmid DNA to recipient cells.
Exo-Fect™ is compatible with larger nucleic acid cargos, including mRNA and plasmid DNA. (Left panels) Exosomes were loaded with 1 µg of mRNA encoding red fluorescent protein (RFP) using Exo-Fect™ and subsequently incubated with HEK293 cells. RFP expression was detected 24 hours post-treatment, indicating successful mRNA delivery and translation. (Right panels) Exosomes were loaded with 5 µg of GFP-encoding plasmid DNA using Exo-Fect™ and added to HEK293 cells. GFP expression was observed 48 hours post-treatment, confirming effective plasmid delivery and gene expression. The presence of RFP and GFP fluorescence in recipient cells demonstrates efficient cargo transfer mediated by Exo-Fect™-loaded exosomes.
Exo-Fect efficiently loads small molecules into exosomes for delivery to recipient cells
Figure 4. Exo-Fect™ enables delivery of small-molecule cargo via exosomes.
Exosomes were loaded with the small molecule cumate using Exo-Fect™ and subsequently incubated with cells harboring a cumate-inducible GFP reporter construct. GFP expression was observed only in cells treated with cumate-loaded exosomes, demonstrating successful encapsulation and delivery of small-molecule cargo mediated by Exo-Fect™.