ExoQuick® Exosome Isolation and RNA Purification kit for Serum & Plasma size: 20 Preps
The ExoQuick Exosome Isolation and RNA Purification Kit (20 preps) provides a complete workflow to isolate high-quality exosomes and extract their RNA from serum and plasma samples. This all-in-one kit combines ExoQuick’s proven exosome precipitation chemistry with an integrated RNA purification step, delivering clean, intact exosomal RNA suitable for downstream molecular analyses.
*2-in-1 workflow : Isolate exosomes and purify RNA from the same sample.
*Optimized for serum & plasma : Consistent yields even from low-volume samples.
*High-quality exosomal RNA : Suitable for qPCR, small RNA analysis, NGS and miRNA profiling.
*No ultracentrifuge required : Simple precipitation-based protocol using standard benchtop centrifuges.
*Fast, reliable workflow : Minimal hands-on time with reproducible results.
*Ideal for liquid biopsy : Enables analysis of circulating exosomal RNA biomarkers.
*Compatible with many downstream assays : RT-qPCR, RNA-Seq, ddPCR, microarrays, EV characterization.
*2-in-1 workflow : Isolate exosomes and purify RNA from the same sample.
*Optimized for serum & plasma : Consistent yields even from low-volume samples.
*High-quality exosomal RNA : Suitable for qPCR, small RNA analysis, NGS and miRNA profiling.
*No ultracentrifuge required : Simple precipitation-based protocol using standard benchtop centrifuges.
*Fast, reliable workflow : Minimal hands-on time with reproducible results.
*Ideal for liquid biopsy : Enables analysis of circulating exosomal RNA biomarkers.
*Compatible with many downstream assays : RT-qPCR, RNA-Seq, ddPCR, microarrays, EV characterization.
Steps of exosome isolation using kits like ExoQuick®
Centrifugation to Remove Cell Debris: Spinning serum or plasma to eliminate cellular debris.
ExoQuick Addition and Incubation: Transferring the supernatant to a new tube, adding ExoQuick reagent, and incubating to precipitate extracellular vesicles (EVs).
Centrifugation for Pellet Collection: Spinning to collect the EV pellet.
Supernatant Removal: Discarding the supernatant to isolate the pellet.
Pellet Resuspension in Buffer: Resuspending the EV pellet in a suitable buffer.
Column Purification: Applying the EV suspension to the ExoQuick Ultra column and spinning to collect purified exosomes.